After some talk in another thread about the possibility of glutamine effecting pumps in the gym, particularly when we are consuming NO boosters to get massive pumps, I decided to look further into the discussion in some medical journals.http://jpet.aspetjournals.org/content/281/1/448.short
The article posted in the link above is titled : "Glutamine Inhibits Nitric Oxide Synthesis in Bovine Venular Endothelial Cells"
From the abstract I will post the key parts : The addition of l-glutamine (0.5 and 2 mM) markedly increased intracellular concentrations of l-glutamine, l-glutamate andl-aspartate and decreased NO synthesis by EC in a concentration-dependent manner in the presence or absence of A23187, bradykinin or substance P
In other words, when they introduced L-glutamine into the experiment, it decreased NO synthesis. The research went further to state that L-glutamine didn't have a negative effect on L-Arganine uptake, and the process involved in decreasing NO synthesis remained unknown after concluding the experiment.
Also in 2004, another study showed that L-glutamine inhibits vascular nitric oxide (NO) production in vitro. (ScienceDirect.com)
So there are all the facts, now here is my anecdotal evidence.
I always consume Xtend throughout the day, and especially during my workout. I usually use 4 scoops, which equals to about 10 grams of glutamine per bottle that I make. I also take a protein shake before my workout which contains 5 grams of glutamine, and also consume amiNO energy by ON during my workout which contains an undisclosed amount of glutamine.
Today I decided to only conume my Xtend for breakfast, and not have my protein shake before my workout or taking a intra-workout drink with me. I took 6 capsules of NO3 Black Chrome 1 hour before my workout along with some Beta-Alanine and went to the gym. I had some of the best pumps I have ever had in months. I couldn't believe it. This was my 6th day taking NO3 Black Chrome, so I know it wasn't the fact that I was using it for the first time. My arms were swole as heck, and my veins were popping out so badly that I was almost scared. Needless to say, I had a great Chest/Bicep workout, and it was amazing.
Tomorrow I'm going to try the same thing with Nitrox II which hasn't let me down and is on par with NO3 Black Chrome.
Now I just want to go and say that even with all the glutamine I consume on an average day and take a pump product, I still get pumps, so some are going to wonder whats the point. But I'm telling you, the difference was outstanding. I'm now only going to conume glutamine after my workouts, when I think it's use is most beneficial anyways.
So give it a try guys. This was only my first test of this idea, and I'll continue to update you guys as I try this out more often.